Research Paper Volume 15, Issue 10 pp 4182—4201

Figure 3. Up-regulation of Sig1R was closely associated with more aggressive clinicopathological features in bladder. (A) One-way ANOVA analysis showed that Sig1R expression levels significantly correlated with the TNM stage in the TCGA BC RNAseq dataset. (B) TCGA BC RNAseq dataset indicated that Sig1R was positively associated with PCNA in bladder cancer tissues. (C) The Kaplan-Meier plot of overall survival in the TCGA cohort is shown according to Sig1R expression. (D) Human bladder epithelial SV-HUC-1 cell, four bladder cancer cell lines and eight paired bladder cancer tissues, and matched adjacent non-tumor tissues (ANT) were collected to evaluate the Sig1R protein expression by immunoblot. GAPDH was used as a loading control. (E, F) Examination of Sig1R expression in bladder cancer and normal tissues by IHC using the H-score approach, scale bar = 100 μm. (G) Examination of Sig1R expression in 40 paired bladder cancer tissues and ANT by IHC using the H-score approach. (H) Sig1R expression and localization in SV-HUC-1 and bladder cancer cell line (T24 and J82) were detected by immunofluorescence, scale bar = 20 μm. **p < 0.01, ***p < 0.001.