Research Paper Volume 15, Issue 10 pp 4182—4201
Sig1R activates extracellular matrix-induced bladder cancer cell proliferation and angiogenesis by combing β-integrin
Figure 7. Sig1R is associated with β-integrin. (A) Co-IP analysis showed the interaction between Sig1R and β-integrin in T24 and J82 cells. Sig1R and β-integrin were immunoprecipitated using antibody against β-integrin. IgG was used as a negative control. (B) Immunofluorescence staining analysis showed the colocalization of Sig1R and β-integrin in T24 and J82 cells, scale bar = 20 μm. (C) Representative images of Duolink In Situ PLA show that there is a direct interaction between Sig1R and β-integrin, and the effect of the two is significantly enhanced after BEM hydrogel coating (orange point), scale bar = 20 μm.