Research Paper Volume 15, Issue 10 pp 3984—4011

Figure 2. eCLIP-seq of G3BP1 and G3BP2 in sodium arsenite (AS)-treated SH-SY5Y cells. (A) Silver staining of immunopurified G3BP1 and G3BP2 and ³²P autoradiogram in SH-SY5Y cells before and after AS treatment. n.i.: non-immune IgG used as an IP negative control. Arrows and square brackets indicate immunopurified proteins and radioactively labelled RNAs, respectively. (B, C) Pie chart depicting the relative contribution of gene categories for G3BP1- (B) and G3BP2-bound RNAs (C). (D) Bar plot showing G3BP1 and G3BP2 preferentially associates with 3′UTR of the target mRNA. (E) Motifs enriched within eCLIP-peaks of G3BP1- and G3BP2-bound RNAs. (F) Intersection of eCLIP-peaks across G3BP1- and G3BP2-bound RNAs before (Control, Ctrl) and after AS treatment.