Research Paper Volume 15, Issue 10 pp 4363—4373

Figure 4. Suppressing STAT1 inhibited Th1 cell differentiation. (A, B) FCM (n=3). Cirsilineol suppressed Th1 cell differentiation. The Th1 cells proportion remarkably decreased compared with those of PCSK6-OV, PCSK6-Si and Control groups. ^*P<0.05 vs. Control group, ^#P<0.05 vs. PCSK6-OV group. (C) ELISA (n=3). IL-2, IFN-γ, TNF-α, IL-6 and IL-1β contents of Cirsilineol group decreased relative to Control and PCSK6-OV groups, with no significant difference versus PCSK6-Si group. ^*P<0.05 vs. Control group, ^#P<0.05 vs. PCSK6-OV group. (D, E) Relative protein expression (n=3). IFN-γ, p-STAT1 and T-bet levels in Cirsilineol group remarkably decreased relative to PCSK6-OV group, with no significant difference versus PCSK6-Si group. ^*P<0.05 vs. Control group, ^#P<0.05 vs. PCSK6-OV group.