Research Paper Volume 15, Issue 10 pp 4391—4410

Figure 3. RNAi-induced knockdown of Bmi-1 inhibited the in vitro proliferation and in vivo tumorigenesis of NPC cells. (A) The relative mRNA levels of Bmi-1 in shBmi-1-expressing 5-8F and SUNE1 cells were determined via qRT-PCR. SCR: scrambled control shRNA. (B) The protein levels of Bmi-1 in shBmi-1-expressing 5-8F and SUNE1 cells were determined via Western blotting. (C, D) A CCK8 assay was employed to assess the growth of shBmi-1-expressing 5-8F and SUNE1 cells. (E, F) A colony formation assay was used to examine the proliferation abilities of shBmi-1-expressing 5-8F and SUNE1 cells. (G, H) Propidium iodide staining and flow cytometry were used to detect the cell cycle distributions of shBmi-1-expressing 5-8F and SUNE1 cells (G), and the statistical results were calculated (H). (I–K) Bmi-1 knockdown inhibited tumor growth from 5-8F cells in nude mice. A representative tumor picture is shown (I), along with a tumor volume growth curve (J) and the tumor weights (K).