Research Paper Volume 15, Issue 10 pp 4391—4410
Hairy gene homolog increases nasopharyngeal carcinoma cell stemness by upregulating Bmi-1
Figure 4. RNAi-induced suppression of Bmi-1 reduced NPC cell stemness. (A) qRT-PCR analysis of various genes in shBmi-1-expressing 5-8F and SUNE1 cells. (B, C) Depletion of endogenous Bmi-1 in 5-8F cells inhibited tumor sphere formation. (D) Western blotting results of cell extracts from shBmi-1-expressing 5-8F and SUNE1 cells. The loading control was GAPDH.