Figure 3. The profiles of lncRNA H19, miR-194, and E2F3 in prostate cancer tissues and cell lines. (A, B) qRT-PCR detected lncRNA H19 expression in prostate cancer tissues and cell lines. (C, D) qRT-PCR determined miR-194 expression in prostate cancer tissues and cell lines. (E, F) qRT-PCR examined E2F3 mRNA expression in prostate cancer tissues and cell lines. (G, H) Western blot was performed for assaying E2F3 protein level. (I) Immunohistochemistry checked E2F3 expression in prostate cancer tissues. Scale bar = 50 μm. *P < 0.05, **P < 0.01, ***P < 0.001 (vs. Normal or PrEC), n = 3.