Research Paper Volume 15, Issue 10 pp 4411—4428

Figure 7. Brevilin A hampered the pro-cancer function of lncRNA H19. Brevilin A was utilized to treat DU145 cells stably transfected with lncRNA H19 overexpression plasmids. (A) CCK8 assay checked cell proliferation. (B) Transwell assay examined cell migration and invasion. Scale bar = 100 μm. (C) TUNEL staining detected cell apoptosis. Scale bar = 50 μm. (D, E) qRT-PCR examined the profiles of lncRNA H19 and miR-194. (F) Western blot was used for examining E2F3 protein level. ^**P < 0.01, ^***P < 0.001 (vs. NC); ^&P < 0.05, ^&&P < 0.01, ^&&&P < 0.001 (vs. H19), n = 3.