Research Paper Volume 15, Issue 10 pp 4481—4497

Figure 2. Changes in neutrophil subsets and expression level of IL-1β gene in peripheral blood of mice after I/R. Results of single-cell sequencing of peripheral blood neutrophils of mice: (A, B) Change trends and percentages of neutrophil subsets in peripheral blood of mice after ischemia/reperfusion (I/R). M0, M1, and M3 represent the mice in the experimental group whose blood was collected after cerebral ischemia and 24 and 72 h after cerebral I/R, respectively; S0, S1, and S3 represent sham-operated mice at the corresponding time points. It can be seen that neutrophil subset 4 in the peripheral blood of mice initially increased and then decreased after I/R. (C, D) Gene expression of IL-1β in different subsets of mouse peripheral blood neutrophils. (C) The percentage of neutrophils expressing IL-1β is the highest in neutrophil subset 4; (D) The IL-1β gene expression level of neutrophil subset 4 is considerably higher than that of other subsets.