Figure 4. LINC00324 knockdown promotes the proliferative ability of MCF-7 cells. (A) qRT-PCR assays for LINC00324 levels in MCF-7 cells transfected with siRNA targeting LINC00324. (B) MCF-7 cells proliferation was detected by MTT assay after LINC00324 knockdown. (C) Transwell assays performed with MCF-7 cells transfected with LINC00324 siRNA or with negative control siRNA. (D) Wound healing assay was performed to determine the migration ability of MCF-7 cells after being transfected with LINC00324 siRNA or with negative control siRNA. (E) Flow cytometry analysis of the percentage of apoptotic MCF-7 cells with LINC00324 knocked-down. * P < 0.05, ** P < 0.01, *** P < 0.001. All data are shown as means ± SEM. Data are from three independent experiments (A, B), or are representative of three independent experiments with similar results (C–E).