Research Paper

Figure 3. Golgi staining revealed morphological changes in pyramidal neurons of PFC and hippocampus from P301S Tau mice at different ages. (A, D, G, J) Representative reconstructed images of layer V pyramidal neurons in PFC (A, D) or CA1 hippocampus (G, J) from WT and P301S mice at 3 months (A, G) and 9 months (D, J). Basal dendrites are traced in blue, while apical dendrites are traced in red. Scale = 100 μm. (B, C, E, F) Plots of dendritic branching as measured by Sholl analysis of basal dendrites and apical dendrites of layer V PFC pyramidal neurons from WT and P301S mice at 3 months (B, C, n = 10 neurons/group) and 9 months (E, F, n = 11 neurons/group). (H, I, K, L) Plots of dendritic branching as measured by Sholl analysis of basal dendrites and apical dendrites of CA1 pyramidal neurons from WT and P301S mice at 3 months (H, I, n = 10 neurons/group) and 9 months (K, L, n = 10 neurons/group). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, and ^****p < 0.0001, two-way ANOVA.