Research Paper Volume 2, Issue 11 pp 791—803

Figure 3. Detection of in situ expression of FAK and α_vβ₃. (A) Single and co-cultures were maintained for 24h in 8-well chamber slides. 4×10⁴ of glioma cells in single cultures and 4×10⁴ glioma cells and 1×10⁴ of hUCBSC for co-cultures were seeded in each well. The cells were then processed for immunocytochemistry of FAK. Inset pictures show DAPI. (B) FACS analysis of cells expressing FAK. The single cultures and co-cultures were detached from the plates using Trypsin and gentle scraping followed by centrifugation at 1000rpm for 3min. The cells were then processed and analyzed for FAK expression using a flow cytometer (BD FACSCalibur). In each sample, 5000 cells were analyzed. (C) Detection of in situ expression of α_vβ₃. The cells were processed for immunocytochemistry to check the expression of α_vβ_3. Inset picture showing DAPI. (D) Quantitative estimation based on (C). Bar = 200μm. The results from three separate experiments are expressed as mean + SE. **p<0.01; *p<0.05.