Research Paper Volume 2, Issue 11 pp 791—803

Figure 6. Inhibition of angiogenic molecules by hUCBSC in vivo. Immunoblot analysis for the expression of various angiogenic molecules in the tissue lysates of brains extracted from nude mice. Orthotopic intracranial tumors were established in nude mice by injecting glioma cells (U251 and 5310) and then treating with hUCBSC. Equal amounts of proteins (40μg) from untreated and treated mice brains were loaded on 8-14% gels and transferred onto nylon membranes, which were then probed with respective antibodies. GAPDH was used a positive loading control. (A, C & E) Western blots showing the downregulation of various angiogenic molecules by hUCBSC treatment. (B, D & F) Quantitative estimations of (A), (C) & (E). (G) Downregulation of various angiogenic molecules at the RNA level by hUCBSC. β-actin expression was used to serve as a loading control. The results from three separate experiments are expressed as mean + SE. **p<0.01; *p<0.05.