Research Paper Volume 2, Issue 12 pp 969—980

Figure 2. Immunodetection analysis of PaGLO1 and PaGLO2 levels in the wild type (WT) and transgenic glyoxalase strains. A. Total protein extracts isolated from WT, single PaGlo1- and PaGlo2- overexpression mutants (three independent isolates from each PaGlo1_OEx and PaGlo2_OEx, respectively) and the deletion strain ΔPaGlo1 were analyzed using antibodies against PaGLO1 and PaGLO2 after transfer to a PVDF membrane. BTotal protein extracts isolated from WT and double PaGlo1/PaGlo2 overexpression mutants (three independent isolates) were similarly analyzed. Detection of the cytosolic protein PaPRE3 (β1-subunit of the 20S proteasome) and protein staining of the transfer membrane with Coomassie were performed as loading controls. Accession numbers: PaGLO1: B2AQW8 (UniProt); PaGLO2: B2B554 (UniProt); PaPRE3: Pa_5_4560 (Podospora anserinasequencing consortium).