Research Paper Volume 3, Issue 3 pp 223—236
Gain of survival signaling by down-regulation of three key miRNAs in brain of calorie-restricted mice
Figure 4. Bcl-2/Bax ratios in calorie-restricted mouse brain tissue and over-expressed miRNA- transfected cell lines. Western blot analysis of Bcl-2, Bax and alpha-tubulin as normalization control. (A) In CR-fed mouse brain tissue, increased Bcl-2 is observed compared to Ad Lib (AL)-fed mice, while Bax expression stays constant. Increased Bcl-2/Bax ratio is observed in CR-fed mice, compared to Ad Lib-fed mice. Histogram represents ratios of Bcl-2 and Bax expression. (B) In miRNA- (mmu-miR-34a, mmu-miR-30e and mmu-miR-181a-1*) transfected HEK-293 cells, Bcl-2/Bax ratio declines, as Bcl-2 expression is considerably diminished in miRNA-transfected cells, compared to scrambled and vector controls. Histogram represents ratios of Bcl-2 and Bax expression. (C) In line with results in HEK-293 transfected cells, miRNA-transfected NIH/3T3 cells also exhibit a decline in Bcl-2 expression, resulting in a decline of the Bcl-2/Bax ratio. Histogram represents ratios of Bcl-2 and Bax expression. (**p<0.01, **p<0.0001; all histograms represent average ± std.dev.)