Research Paper Volume 3, Issue 3 pp 237—261

Figure 3. Western analysis of hUbb protein expression. Total protein was isolated from 30 male flies, diluted as indicated, fractionated using SDS-PAGE and Western blotted. (A) Control flies and hUbb transgenic strain fly protein incubated with antibody specific for hUbb. The bracket indicates high MW species induced by DOX. (B) Control flies and transgenic strains expressing hUbb or hUbb⁺¹, total protein stain. Purified hUbb protein monomer was run as control. (C) Control flies and transgenic strains expressing hUbb or hUbb⁺¹, incubated with antibody specific for hUbb. Purified hUbb protein monomer is run as control (position indicated by asterisk). Samples are the same as shown in (B). The change in monomer abundance upon DOX treatment was determined using densitometry: control = 0.94; hUbb⁺¹[11] = 0.98; hUbb[70] = 3.2 fold.