Research Paper Volume 3, Issue 10 pp 1003—1017
Ishemia-Reperfusion enhances GAPDH nitration in aging skeletal muscle
Figure 4. PCR analysis of GAPDH transcript levels from control and I/R plantaris muscle following 5 days of reperfusion. PCR with GAPDH specific probes was performed using an equal percentage of total cDNA (reverse transcribed from mRNA using igo(d)T primers) isolated from each sample of (A) young (6-7 months) and (B) old (24-27 months) plantaris muscle. Control = black; I/R = gray; T = Threshold (arrow shows the threshold).