Research Paper Volume 3, Issue 10 pp 1028—1038

Genome protective effect of metformin as revealed by reduced level of constitutive DNA damage signaling


Figure 2. Effect of metformin (MF) on the level of constitutive ATM phosphorylation on Ser1981 in A549 cells. Similar as in Figure 1, the cells were treated with 1, 5 or 20 mM MF for 48 h. Left panels present bivariate distributions of cellular DNA content vs intensity of ATM-S1981P IF. The mean values of ATM-S1981P for cells in G1, S, and G2M were obtained by gating analysis and are shown (+SD) as the bar plots (right panel). The skewed dash line shows the upper level of ATM-S198P IF intensity for 97% of G1- and S- phase cells in Ctrl.