Research Paper Volume 3, Issue 10 pp 1028—1038

Genome protective effect of metformin as revealed by reduced level of constitutive DNA damage signaling


Figure 4. Effect of metformin on the level of constitutive expression of ATM-S1981P. Exponentially growing TK6 cells were untreated (Ctrl) or were grown in the presence of 0.1, 1.0, 5.0 and 10 mM metformin (MF) for 48 h. The expression of ATM-S1981P was detected with phospho-specific Ab. As in Fig. 3, the cells were gated in G1, S and G2M phases of the cell cycle and the mean values of ATM-S1981P for cells in each of these cell cycle phases were estimated. The figures show the percent reduction in mean values of ATM-S1981P IF of the metformintreated cells with respect to the mean values of the untreated cells (Ctrl) in the respective phases of the cell cycle.