Research Paper Volume 3, Issue 10 pp 1028—1038

Figure 5. Effect of metformin on constitutive expression γH2AX and ATM-S1981^P in normal human proliferating lymphocytes. Peripheral blood lymphocytes were mitogenically stimulated by phytohemagglutinin for 48 h and then were grown in the absence (Ctrl) or presence of 5 mM metformin (MF) for additional 24 h. The expression of γH2AX and ATM-S1981^P was detected with phospho-specific Abs and cell fluorescence we as measured by flow cytometry. The numerical figures show the percent reduction in expression of γH2AX and ATM-S1981^P of cells treated with metformin with respect to Ctrl, in the respective phases of the cell cycle.