Research Paper Volume 3, Issue 10 pp 1028—1038

Genome protective effect of metformin as revealed by reduced level of constitutive DNA damage signaling


Figure 5. Effect of metformin on constitutive expression γH2AX and ATM-S1981P in normal human proliferating lymphocytes. Peripheral blood lymphocytes were mitogenically stimulated by phytohemagglutinin for 48 h and then were grown in the absence (Ctrl) or presence of 5 mM metformin (MF) for additional 24 h. The expression of γH2AX and ATM-S1981P was detected with phospho-specific Abs and cell fluorescence we as measured by flow cytometry. The numerical figures show the percent reduction in expression of γH2AX and ATM-S1981P of cells treated with metformin with respect to Ctrl, in the respective phases of the cell cycle.