Research Paper Volume 3, Issue 10 pp 1028—1038
Genome protective effect of metformin as revealed by reduced level of constitutive DNA damage signaling
Figure 6. Effect of metformin on ability of TK6 cells to oxidize 2',7'-dihydro-dichlorofluorescein diacetate (H2DCF-DA). TK6 cells were untreated (Ctrl) or treated with 1, 5 or 10 mM metformin (MF) for 48 h. The cells were then incubated for 30 min with 10 μM H2DCF-DA and their fluorescence was measured by flow cytometry. While H2DCF-DA is not fluorescent, the product of its oxidation (DCF) by intracellular ROS shows strong green fluorescence. Note dramatic decline in fluorescence intensity of cells treated with 5 or 10 mM metformin.