Research Paper Volume 4, Issue 1 pp 3—12
Mitochondrial oxidative stress caused by Sod2 deficiency promotes cellular senescence and aging phenotypes in the skin
Figure 1. Cellular senescence and mitochondrial activity in skin of aging mice.
(A) Representative photomicrographs of dorsal skin sections from C57BL/6J WT mice, aged 4 mos or 2 yrs, stained for SA-βgal activity (blue) and counterstained with nuclear fast red (red). Arrows indicate SA-βgal+ cells. (B) Percent of C57BL/6J WT mice, aged 4 mos, 8 mos or 2 yrs, with SA-βgal positive (+) or negative (−) activity. (C) Representative photomicrographs of dorsal skin sections from the same mice, aged 4 mos or 2 yrs, stained for succinate dehydrogenase (SDH) (blue) activity. Arrows indicate staining in epidermis. (D) Percent of the same mice, aged 4 mos, 8 mos or 2 yrs, with SDH positive (+) or negative (−) activity. A total of six 4-month old (3 males and 3 females), ten 8-month old (5 males and 5 females), and thirteen 24-month old (7 males and 6 females) mice were analyzed.