Research Paper Volume 4, Issue 1 pp 3—12
Mitochondrial oxidative stress caused by Sod2 deficiency promotes cellular senescence and aging phenotypes in the skin
Figure 5. Epidermal thinning in Sod2−/− mice.
(A) Representative photomicrographs of H&E staining of dorsal skin sections from WT (n=8) and Sod2−/− (n=9) mice, aged 17-20 days. (B) Quantitation (means ± SEM) of the thickness of the epidermis (EP) and stratum corneum (SC) in skin sections from WT (n=8) and Sod2−/− (n=9) mice using Image J software. (C) Quantitative PCR analysis of transglutaminase 1 (Tgm1), keratin 10 (Krt10) and keratin 1 (Krt1) mRNA levels in dorsal skin of WT (n=8) and Sod2−/− (n=9) mice, aged 17-20 days. Transcript levels were normalized to beta-actin levels. Means with asterisks indicate significant differences at p<0.05 by Student's t test.