Research Paper Volume 4, Issue 1 pp 40—48
Age-related resistance of skeletal muscle-derived progenitor cells to SPARC may explain a shift from myogenesis to adipogenesis
Figure 1. Immunocytochemistry of SPARC in SMPCs. Immunostaining of SPARC during myogenic and adipogenic differentiation of SMPCs from young rats. Cells were co-stained with anti-MyoD and anti-SPARC 3 days after plating or with anti-MHC and anti-SPARC 4 days after exposure to media containing 2% HS. Co-staining with anti-SPARC and anti-C/EBPα or Oil Red O staining was performed 4 days after culturing in the media to induce adipogenic differentiation (ADM). Nuclei were visualized with Hoechst 33258 Dye (blue). White arrowheads indicate double-positive cells (MyoD+/SPARC+, MHC+/SPARC+, C/EBPα+/SPARC+, and Oil Red O+/SPARC+), whereas black arrowheads indicate cells that are only positive for SPARC (MyoD-/SPARC+, MHC-/SPARC+, C/EBPα-/SPARC+, and Oil Red O-/SPARC+). Scale bar = 100 μm.