Research Paper Volume 4, Issue 1 pp 40—48

Age-related resistance of skeletal muscle-derived progenitor cells to SPARC may explain a shift from myogenesis to adipogenesis

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Figure 6. Effects of exogenously added SPARC on differentiation of SMPCs with diminished levels of SPARC expression.

SMPCs from young and old rats were cultured for 4 days in adipogenic media in the presence of BSA (20 μg/ml) or recombinant SPARC (20 μg/ml) 1 day after transfection with siSPARC. (A) Oil Red-O-positive areas were quantified as pixel values and the ratios to control values are graphed. Data are expressed as mean ± SEM (n = 3, respectively). *p < 0.05 vs. BSA-treated cells. (B) Proportions of MHC-positive cells were calculated and ratios to control values are graphed. Data are expressed as mean ± SEM (n = 3, respectively). *p < 0.05 vs. BSA treated cells.