Research Paper Volume 4, Issue 1 pp 40—48
Age-related resistance of skeletal muscle-derived progenitor cells to SPARC may explain a shift from myogenesis to adipogenesis
Figure 7. Itga5 mRNA and protein expression in young and old rat SMPCs.
(A) qPCR analysis of Itga5 and Itgb1 expression in SMPCs from young and old rats 3 days after plating. Graphed data are expressed as mean ± SEM (Itga5: young; n = 4, old; n = 6, Itgb1: n = 4, respectively). *p < 0.05 vs. young. (B) Typical immunostaining of ITGA5 in SMPCs from young and old rats 3 days after plating. Nuclei were counterstained with Hoechst 33258. White and black arrowheads indicate intensely ITGA5-positive and slightly ITGA5 -positive cells, respectively. Scale bar = 100 μm. (C) Quantitative analysis of relative fluorescence of ITGA5 in young and old rats SMPCs 3 days after plating. Graphed data are mean ± SEM (n = 3, respectively). *p < 0.05 vs. young rats.