Research Paper Volume 4, Issue 1 pp 40—48
Age-related resistance of skeletal muscle-derived progenitor cells to SPARC may explain a shift from myogenesis to adipogenesis
Figure 8. Knockdown of ITGA5 expression in young SMPCs by siRNA transfection.
(A) Efficiency of siRNA silencing was evaluated by qPCR in SMPCs from young rats 2 days after transfection with siItga5: Graphed data are expressed as mean ± SEM (n = 6, respectively). *p < 0.05 vs. control. (B) Quantitative analysis of relative fluorescence of ITGA5 in young SMPCs 2 days after transfection with siItga5. Graphed data are expressed as mean ± SEM (n = 3, respectively). *p < 0.05 vs. control. Quantitative analysis of Oil Red-O staining results (C) and proportion of MHC-positive cells (D) of SMPCs at 5 days after siItga5 or siSPARC and siItga5 cotransfection. Graphed data are expressed as mean ± SEM (n = 3, respectively). Letters (a, b and c) indicate statistically significant differences (p < 0.05). Quantitative analysis of Oil Red-O staining results (E) and proportion of MHC-positive cells (F) of SMPCs cultured for 4 days in the presence of BSA (20 μg/ml) or recombinant SPARC (20 μg/ml) followed by cotransfection with siSPARC and siItga5. Graphed data are expres-sed as mean ± SEM (n = 3, respectively). *p < 0.05 vs. control.