Research Paper Volume 4, Issue 5 pp 368—374
Integrin α5β1 simultaneously controls EGFR-dependent proliferation and Akt-dependent pro-survival signaling in epidermoid carcinoma cells
Figure 4. Integrin α5 knock-down and inhibition of EGFR differently affect expression of cell cycle and apoptosis regulators in A431 cells.
The cells were transduced with control or α5 shRNA-containing vectors or treated with PD168393 (as detailed in “Materials and methods” and Fig. 2) then western blot analysis and ECL-staining were performed. The blots were probed with 1:300 dulution of c-Myc antibodies and with 1:1000 dilution of other antibodies.