Research Paper Volume 4, Issue 7 pp 462—479
Methylation by Set9 modulates FoxO3 stability and transcriptional activity
Figure 1. FoxO3 is methylated by Set9 in vitro.
(A) In vitro methylation of the N-terminal portion of FoxO3 (amino acids 1-300) by 8 different methyltransferases. ◁: FoxO3 methylated by Set9, *: Set9 auto-methylation. (B) In vitro methylation of the C-terminal portion of FoxO3 (amino acids 301-673) by 8 different methyltransferases. *: Set9 auto-methylation. (C) Methylation of the full-length FoxO3 protein by Set9. ◁: full-length FoxO3, ◀: FoxO3 degradation product, *: Set9 auto-methylation.