Research Paper Volume 4, Issue 7 pp 462—479
Methylation by Set9 modulates FoxO3 stability and transcriptional activity
Figure 3. Set9 preferentially methylates FoxO3 among FoxO family members.
(A) In vitro methylation of different mammalian FoxO family members or FoxO orthologs. The FoxO1, FoxO3 and FoxO4 constructs were generated from the human sequence, whereas the FoxO6 construct was generated from the mouse sequence. ◀: FoxO6 degradation product, ←: full-length FoxO6, *: Set9 auto-methylation. (B) Alignment of the region of FoxO3 surrounding K271 to that of other mammalian FoxO family members and FoxO orthologs.