MDL-1, a growth- and tumor-suppressor, slows aging and prevents germline hyperplasia and hypertrophy in <i>C. elegans</i>

Michèle Riesen; Inna Feyst; Nattaphong Rattanavirotkul; Marina Ezcurra; Jennifer M.A. Tullet; Irene Papatheodorou; Matthias Ziehm; Catherine Au; Ann F. Gilliat; Josephine Hellberg; Janet M. Thornton; David Gems

doi:doi:http://dx.doi.org/10.18632/aging.100638

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Research Paper Volume 6, Issue 2 pp 98—117

MDL-1, a growth- and tumor-suppressor, slows aging and prevents germline hyperplasia and hypertrophy in C. elegans

Figure 6. Tests for action of mdl-1 in the intestine.

(A,B) Effect of mdl-1 on yolk accumulation. (A) Example of Coomassie stained gel with C. elegans protein extracts. (B) Bar graph data is derived from densitometric measurement of protein on gels (means of 3 biological replicates; error bars, standard error). It shows levels of the major yolk protein YP170 normalized to myosin levels (which are not expected to change), and again to levels in N2 on day 1. Actin/myosin ratio gives an indication of the reliability of myosin as a standard. * 0.01 < p < 0.05; ** 0.001 < p < 0.01, compared to N2 of the same age. (C) Effects on lifespan of pges-1::mdl-1(+) rescue of mdl-1(0) (for statistics, see Table S3, trial 2).