Research Paper Volume 8, Issue 6 pp 1276—1285

Figure 1. Observation of spontaneous Ca²⁺ transient in A549 cells. A549 cells were loaded with fluo-4 AM, and Ca²⁺ activities were recorded using confocal microscopy. (A) Ca2+ transients recorded in the xy-t mode. (B) No calcium signalling was observed in the leading area with relatively low calcium concentration. The arrows indicate ruffling pseudopodium. (C) Long-term (120 min) recording of A549 cell migration in A549 cells detected using the calcium indicator protein GCaMP J. The scanning images were recorded using an API live cell imaging system with pseudo-colour treatment (60× objective lens, 512×512 pixels, XYT scanning, 5 s/frame).