Research Paper Volume 8, Issue 6 pp 1276—1285

Figure 2. Ca²⁺ signalling regulates A549 cell migration by affecting cell adhesion. (A) The DIC images show changes in cell adherence during the time lapse under different conditions. (b) 5 mM EGTA in extracellular solution inhibited A549 cell adhesion compared to the control (a) and the inhibitory effect of EGTA on cell adhesion was abolished by EGTA washout (c). The bar graphs (d) show the relative cell area calculated for statistical analysis. (B) The IP3Rs inhibitor 2-APB inhibits A549 cell migration via the inhibition of Ca²⁺ signalling. 30 μM 2-APB inhibited ATP-induced calcium release via IP3Rs (a and b) and significantly inhibited wound healing in A549 cells (C and D).