Research Paper Volume 8, Issue 6 pp 1276—1285

Figure 3. Inhibition of Ca²⁺ signalling inhibits A549 cell migration by affecting cell polarization. (A) Images show that the polarization and pseudopodium protrusion of A549 cells were inhibited in the presence of 2-APB (30 μM). (B) Treatment with 2-APB during the latter half of the assay did not affect wound healing. (C) Real-time recording of A549 cells' random motility. The sequential pictures were shown to explain the computing method. The centre of gravity was calculated based on the outlines of the cells (red circle), and the change in the coordination of the centre represented the tracking of cell movement. (D) Statistical analysis of the migration rate for each cell. The x-axis represents the cell rates during the initial 1.5 h, and the y-axis represents the cell rates during the latter 1.5 h. The dots in the lower right diagonal represent the cells with decreased rates.