Research Paper Volume 8, Issue 6 pp 1276—1285

Figure 4. ERP44 inhibits cell migration by reducing intracellular Ca²⁺ release. (A) Identification of ERP44 overexpression (ERP44-OE) system in A549 cells via western blot and immunofluorescence. Overexpressed ERP44 were co-located with ER marker Bip. (B) ERP44 overexpression inhibited 10 μM ATP-induced calcium release via IP3Rs. (C) Wound healing was significantly inhibited by overexpressed ERP44. (D) Overexpression of ERP44 inhibited A549 cells random motility. A549 cells were recorded in real time after adenovirus infection. Circled cells are DsRed-positive cells. The right panel shows the movement tracking of A549 cells.