Research Paper Volume 8, Issue 12 pp 3298—3310
miR-34a is a common link in both HIV- and antiretroviral therapy-induced vascular aging
Figure 6. Sirt1 is a direct target gene of miR-34a that is related to HIV-Tat protein and antiretroviral agents-induced vascular aging. (A) pmiR-miR-34a, but not pmiR-31 or pDNR-CMV, inhibited luciferase activity. (B) the expression of Sirt1, was increased by miR-34a knockdown via antagomiR-34a (30 nM), but was decreased by miR-34a over expression via Ad-miR-34a (30 MOI). (C) The expression of Sirt1 in ECs was decreased by HIV-Tat protein or antiretroviral agents. (D) miR-34a-mediated senescence of cultured ECs was partially inhibited by the protector of Sirt1(30 nM). Note: n=6; *p<0.05 compared with control groups.