Accumulation of spontaneous γH2AX foci in long-term cultured mesenchymal stromal cells

Margarita Pustovalova; Anna Grekhova; Тatiana Astrelina; Viktoria Nikitina; Ekaterina Dobrovolskaya; Yulia Suchkova; Irina Kobzeva; Darya Usupzhanova; Natalia Vorobyeva; Aleksandr Samoylov; Andrey Bushmanov; Ivan V. Ozerov; Alex Zhavoronkov; Sergey Leonov; Dmitry Klokov; Andreyan N. Osipov

doi:doi:10.18632/aging.101142

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Research Paper Volume 8, Issue 12 pp 3498—3506

Accumulation of spontaneous γH2AX foci in long-term cultured mesenchymal stromal cells

Figure 1. Immunocytochemical analysis of γH2AX and pATM foci. (A) Changes in γH2AX, pATM foci and their co-localization depending on the passage number in MSCs. (B) Comparative analysis of γH2AX, pATM foci and their co-localization in early (3-8) vs. late (18-22) passages of MSCs. (C) Fraction of γH2AX foci that co-localize with pATM at early (3-8) vs. late (18-22) passages of MSCs. (D) Representative immunofluorescent microphotographs of MSC showing γH2AX (green), pATM (red) foci and their co-localization (yellow) at passage 5 and 20. Nuclei were counterstained with DAPI.