Research Paper Volume 8, Issue 12 pp 3535—3551

Figure 1. (A) Cytostatic effect. Effect of TOR inhibitors (Ti) on proliferation. HT-p21 cells were treated with serial dilutions of indicated Ti for 4 days and counted in triplicates. Data presented as mean ± SD. (B) Reversibility. Cells were treated as in A. After 4 day-treatment cells were counted and re-plated at 1000/well in 6-well plates in drug-free medium. Cells were allowed to re-proliferate for 4 days and counted. Cytostatic arrest was fully reversible. (C) Gerosuppression. Effect of TOR inhibitors on re-proliferative potential. HT-p21 cells were treated with IPTG in the presence of different concentrations of indicated Ti in triplicates. After 4 day-treatment, cells were washed off the drugs and allowed to regrow in drug-free medium for 7 days and counted. Data presented as mean ± SD.