Research Paper Volume 8, Issue 12 pp 3535—3551

Figure 6. Effect of TOR inhibitors on chronological senescence of cancer HT-p21 cells. Cells were plated at high density in 96-well plates and treated with TOR inhibitors or rapamycin at selected optimal concentrations. After 3 days in culture cells were photographed (color manifests pH of medium), trypsinized and small aliquots were re-plated in 6-well plates. Formed colonies were stained after 7 days in culture with Crystal Violet.