Research Paper Volume 9, Issue 6 pp 1607—1622

Figure 3. Quantification of C6S after chondro-6-sulfatase digestion. (A) A composite picture taken from two gels, one with buffer 1 and the other with buffer 4. Each extract shows the ChABC digested lane and the same extracted digested with chondro-6-sulfatase. (B and C) Quantification of C6S levels in buffer 1 and buffer 4 extract. There is a significant reduction in C6S at 12m and 18m time points in buffer 4 fractions. (D) The ratio of C4S to C6S at the four time points, plotted respectively from the data in figure 2 and 3. The ratio significantly increases in favor of the inhibitory C4S in 12 and 18 months PNNs extracts.

Graphs show mean ± s.e.m. *** indicates a significant relationship between the considered CS GAGs and time by One Way Anova, p >0.001.** indicates a significant relationship between the considered ratio and time by Kruskal-Wallis test, p =0.002.B1, ECM fraction; B4, PNNs fraction; B1+B2+B3+B4, sum of GAGs from the four fractions of each brain; 3m, 3-month-old brains; 6m, 6-month-old brains; 12m, 12-month-old brains; 18m, 18-month-old brains; C4S, Chondroitin-4-sulfate; C6S, Chondroitin-6-sulfate; C0S, Chondroitin non-sulfated; HA, hyaluronic acid.