Research Paper Volume 10, Issue 3 pp 463—480

Figure 4. Changes about percent of germ cell in cyst and follicle in nicotine-exposed and melatonin-treated ovaries after 5 days culture in vitro. (A) Percentage of oocytes in cyst and follicle after the ovaries treated with nicotine at incresing dosage (5 μM-500 μM) for 5 days culture in vitro. (B) Number of oocytes in one section after the ovaries treated with nicotine at incresing dosage (5 μM-500 μM) for 5 days culture in vitro. (C) Number of primordial follicles in one section after 5 days culture in vitro of control, 10 μM nicotine, 100 μM nicotine and 10 μM nicotine plus 1 μM melatonin. (D) Representative IF for the germ cell marker Mvh in ovary tissue sections after 5 days culture in vitro of control, 10 μM nicotine, 100 μM nicotine and 10 μM nicotine plus 1 μM melatonin. (E) Percentage of oocytes in cyst and follicle after 5 days culture in vitro with 10 μM nicotine plus 0.5 μM, 1 μM and 5 μM melatonin respectively. (F) Number of oocytes in one section after 5 days culture in vitro with 10 μM nicotine plus 0.5 μM, 1 μM and 5 μM melatonin respectively. The results are presented as mean±SD. All the experiments were repeated at least three times. * P < 0.05; ** P < 0.01.