Research Paper Volume 10, Issue 5 pp 1103—1132

Figure 5. Changes in miRNA composition of senescent cell derived sEVs. (A) Volcano plot shows 31 significantly differently present senescence-associated (SA) sEV-miRNAs after normalization to the global means at D7 and (B) 32 SA sEV-miRNAs at D21 after the last H₂O₂ treatment. (C) Venn diagram shows miRNAs more abundantly present in sEVs of SIPS cells. (D) Venn diagram shows miRNAs less abundant in sEVs of SIPS cells. (A-B) Raw Ct-values from each sample were normalized to the respective global mean. Log2FC of SIPS relative to Q control cells were calculated. Values from D7 (panel A) and D21 (panel B) recovery are plotted on x-axis against their individual -log10(p-value) on y-axis. Horizontal dotted lines indicate a separation between miRNAs passing a p-value higher or lower than 0.05. Vertical dotted lines separate secreted miRNAs with log2FC > 1 or log2FC < 1. MiRNAs reaching a p-value < 0.05 are illustrated with green and blue dots and miRNAs with a p-value > 0.05 are shown in black. None reached the 0.05 cut-off value for the FDR of an adjusted p-values. Analysis was performed using three different HDF cell strains (n = 3) each Q and SIPS from two different time points (D7 and D21). (C-D) Log2FC was calculated and significantly regulated (p-value < 0.05) miRNAs from D7 and D21 were compared in a Venn diagram.