Research Paper Volume 10, Issue 8 pp 2062—2078

Figure 2. BANCR knockdown suppressed invasion, proliferation, induced apoptosis and increased ADR sensitivity in CRC cells. (A) Expression of BANCR in human normal colon mucosal epithelial cell line (NCM460) and CRC cell lines (LoVo and HCT116) was detected using RT-qPCR assay. (B-K) LoVo and HCT116 cells were transfected with si-Control or si-BANCR with untransfected (NC) or si-Control-transfected cells acted as blank or mock control, respectively. (B and C) Knockdown efficiency of si-BANCR was evaluated by RT-qPCR assays at 48 h upon transfection. (D and E) The effect of BANCR depletion on proliferation ability was measured by MTT assay at the indicated time points (0, 24, 48, 72 h) upon transfection in LoVo and HCT116 cells. (F and G) The effect of BANCR knockdown on invasion capability was assessed at 48 h after transfection by transwell invasion assay in LoVo and HCT116 cells. (H and I) The effect of BANCR deficiency on apoptotic rate was detected in LoVo and HCT116 cells at 48 h posttransfection by flow cytometry via double-staining of Annexin-V-FITC and PI. (J and K) LoVo and HCT116 cells were treated with different concentrations of ADR (0, 20, 40, 80, 160, 320, 640, 1280 ng/ml) for 48 h, followed by the determination of cell survival rate using MTT assay. *P < 0.05.