Research Paper Volume 10, Issue 8 pp 2062—2078

Figure 3. CSE1L downregulation resulted in a reduction of invasion and proliferation capacities, and an increase of apoptosis rate and ADR sensitivity in CRC cells. (A and B) CSE1L expressions at mRNA and protein levels were measured by RT-qPCR and western blot assays in NCM460, LoVo and HCT116 cells. (C) LoVo and HCT116 cells were transfected with si-Control or si-CSE1L, followed by measurement of CSE1L protein level via western blot assay at 48 h upon transfection. (D and E) The effect of CSE1L silencing on proliferation was assessed by MTT analysis in LoVo and HCT116 cells. (F and G) The effect of CSE1L knockdown on invasion was detected via transwell invasion assay in LoVo and HCT116 cells. (H and I) The effect of CSE1L deficiency on apoptotic rate was tested by flow cytometry in LoVo and HCT116 cells. (J and K) LoVo and HCT116 cells were transfected with si-Control or si-CSE1L for 24 h, then untransfected or transfected cells were treated with different concentrations of ADR (0, 20, 40, 80, 160, 320, 640 and 1280 ng/ml) for another 48 h, followed by the detection of cell survival rate using MTT assay. *P < 0.05.