Research Paper Volume 10, Issue 10 pp 2723—2740
Long-term intake of Lactobacillus paracasei KW3110 prevents age-related chronic inflammation and retinal cell loss in physiologically aged mice
Figure 2. Intake of Lactobacillus paracasei KW3110 suppressed the inflammatory CD4-positive T cell expansion in the lamina propia of the small intestine (SI-LP). (A and B) To detect inflammatory cytokine-producing cells, SI-LP cells from young mice (3-months-old) and aged mice (17-months-old) were cultured under stimulation with Leukocyte Activation Cocktail plus BD GolgiPlug, and analyzed by flow cytometry. (A) Representative data of CD4-positive cells from aged mice fed a diet with (KW3110) or without (CTL) L. paracasei KW3110. (B) The ratio of CD3ε- and CD4-positive to live cells. (C) Representative data of CD4- and interferon gamma (IFN-γ)-positive cells from aged mice fed a diet with or without L. paracasei KW3110. (D) The ratio of CD4- and IFN-γ-positive cells to CD4-positive cells. (E) The expressions of programmed cell death protein 1 (PD-1) in CD3ε- and CD4-positive cells were analyzed by flow cytometry. M.F.I. indicates mean fluorescence intensity.