Mus Musculus) can be slowed by gold standard anti-aging interventions such as calorie restriction and growth hormone receptor knock-outs. Using DNA methylation data from previous publications with data collected in house for a total 1189 samples spanning 193,651 CpG sites, we developed 4 novel epigenetic clocks by choosing different regression models (elastic net- versus ridge regression) and by considering different sets of CpGs (all CpGs vs highly conserved CpGs). We demonstrate that accurate age estimators can be built on the basis of highly conserved CpGs. However, the most accurate clock results from applying elastic net regression to all CpGs. While the anti-aging effect of calorie restriction could be detected with all types of epigenetic clocks, only ridge regression based clocks replicated the finding of slow epigenetic aging effects in dwarf mice. Overall, this study demonstrates that there are trade-offs when it comes to epigenetic clocks in mice. Highly accurate clocks might not be optimal for detecting the beneficial effects of anti-aging interventions." name="description"> A multi-tissue full lifespan epigenetic clock for mice - Figure f5 | Aging
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Research Paper Volume 10, Issue 10 pp 2832—2854

A multi-tissue full lifespan epigenetic clock for mice

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Figure 5. Genome-wide association results for DNAm Age. (a) Manhattan plot presenting genome-wide association results for DNAm Age. Epigenetic age predictions were calculated using all CpGs clock with ridge regression and leave-one-sample-out estimates. GWAS analysis was based on linear mixed model and a set of 196,148 SNPs (MAF > 0.05) from HMDP mice strains. (b) This SNP as identified using GWAS analysis of epigenetic age predictions. It is located in an LD block on chromosome 6 and contains the genes Npy, Mpp6, Gsdme and Osbp13. A one-sided t-test of DNAm ages between the two allelic groups shown is statistically significant. (c) It is located in an LD block on chromosome 6 and contains the genes Npy, Mpp6, Gsdme and Osbp13. A one-sided t-test of DNAm ages between the two allelic groups shown is statistically significant.