Research Paper Volume 10, Issue 11 pp 3148—3160

Figure 6. Percentage of senescent cells. (A) Representative micrographs of isolated hDF obtained from young, elderly and HGPS donors. Cells with blue staining indicated positive for SA β-galactosidase activity. Images acquired in transmission light bright field at 400× magnification. Scale bar: 10 μm. (B) The percentages of positive hDF from Young, Elderly and HGPS groups are reported in the graph as mean value of three independent staining (% ± STD). Significant differences calculated according to the Student’s t-test (p<0.05) are indicated.