Research Paper Volume 10, Issue 11 pp 3371—3381

Figure 6. CDKN2BAS down-regulates KLF13 expression and inhibits EPI-induced apoptosis. (A) The expression of caspases and cleaved PARP after EPI treatment was detected by Western blot; (B) The effect of CDKN2BAS overexpression on the phosphorylation of ERK and MEK in HCCLM3 cells was detected by Western blot; (C-D) The effect of CDKN2BAS overexpression or knockdown on KLF13 mRNA expression was detected by qRT-PCR; (E) EPI–induced apoptosis was detected by flow cytometry in HCCLM3 cells transfected with CDKN2BAS siRNA and/or KLF13 siRNA; (F) Cell proliferation of HCCLM3 cells was detected by MTT after siRNA transfection.