Research Paper Volume 10, Issue 11 pp 3382—3396
Greater loss of mitochondrial function with ageing is associated with earlier onset of sarcopenia in C. elegans
Figure 5. Longevity at lower cultivation temperatures is associated with lower mitochondrial function and lower mitochondrial content. (A) At the onset of young adulthood, MAPR in worms with GFP-labelled mitochondria was significantly lower at 15°C, and greater at 25°C than at 20°C. GS denotes glutamate and succinate; GM denotes glutamate and malate; PyM denotes pyruvate and malate; PalM denotes palmitoyl-L-carnitine and malate; and S denotes succinate as respiratory substrate (n = 6, P<0.05 – P<0.001). (B) Maximal CS activity, indicative of mitochondrial content, was lowest at 16°C and highest at 25°C (n = 8, P<0.01). (C) Lipid peroxidation was not significantly different between animals at different temperatures (n = 6 – 11, P >0.05). Data represent mean ± SEM, * P<0.05; ** P<0.01; *** P<0.001.