Figure 4. The effects of heterochronic parabiosis on the contents of iron and the expression of iron metabolism proteins in the kidney of mice. The contents of iron (A), and TfR1, Fpn1, Ft-H, Ft-L and CP proteins (B – E) in the kidney were determined in Y-Y, Y-O(Y), O-O and Y-O(O) mice using western blot analysis or the methods described previously. Data are presented as means ± SEM (n=4). *p<0.05 and **p<0.01 vs. the control.